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rat pulmonary artery endothelial cells rpaec  (PromoCell)


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    PromoCell rat pulmonary artery endothelial cells rpaec
    Proliferative characteristics of bone marrow-derived EPC . EPC and <t>rPAEC</t> <t>were</t> <t>cultured</t> in the presence of 20% FCS in EBM-2 without further supplements (Figure 3A) or in EBM-2 containing bFGF (Figure 3B). Figure 3A shows the differential growth capacities of EPC and mature rPAEC in the presence of EBM-2 supplemented with 20% FCS. Figure 3B shows the proliferative kinetics of EPC cultured in EBM-2 supplemented with 0.5% FCS towards increasing concentrations of bFGF (100 ng/ml to 100 μg/ml). Cells were seeded in 96-well plates, grown for 24 h in culture medium, washed twice with HEPES/EDTA and treated with bFGF in EBM-2 medium containing 0.5% FCS for 48 h as indicated. Cells were incubated with MTT solution, lysed and the absorbance was read. MTT activity is expressed as Optical Density and represents mean ± SEM of five independent experiments, ** p < 0.01, * p < 0.05.
    Rat Pulmonary Artery Endothelial Cells Rpaec, supplied by PromoCell, used in various techniques. Bioz Stars score: 99/100, based on 2482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat pulmonary artery endothelial cells rpaec/product/PromoCell
    Average 99 stars, based on 2482 article reviews
    rat pulmonary artery endothelial cells rpaec - by Bioz Stars, 2026-03
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    1) Product Images from "Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury"

    Article Title: Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury

    Journal: Respiratory Research

    doi: 10.1186/1465-9921-8-50

    Proliferative characteristics of bone marrow-derived EPC . EPC and rPAEC were cultured in the presence of 20% FCS in EBM-2 without further supplements (Figure 3A) or in EBM-2 containing bFGF (Figure 3B). Figure 3A shows the differential growth capacities of EPC and mature rPAEC in the presence of EBM-2 supplemented with 20% FCS. Figure 3B shows the proliferative kinetics of EPC cultured in EBM-2 supplemented with 0.5% FCS towards increasing concentrations of bFGF (100 ng/ml to 100 μg/ml). Cells were seeded in 96-well plates, grown for 24 h in culture medium, washed twice with HEPES/EDTA and treated with bFGF in EBM-2 medium containing 0.5% FCS for 48 h as indicated. Cells were incubated with MTT solution, lysed and the absorbance was read. MTT activity is expressed as Optical Density and represents mean ± SEM of five independent experiments, ** p < 0.01, * p < 0.05.
    Figure Legend Snippet: Proliferative characteristics of bone marrow-derived EPC . EPC and rPAEC were cultured in the presence of 20% FCS in EBM-2 without further supplements (Figure 3A) or in EBM-2 containing bFGF (Figure 3B). Figure 3A shows the differential growth capacities of EPC and mature rPAEC in the presence of EBM-2 supplemented with 20% FCS. Figure 3B shows the proliferative kinetics of EPC cultured in EBM-2 supplemented with 0.5% FCS towards increasing concentrations of bFGF (100 ng/ml to 100 μg/ml). Cells were seeded in 96-well plates, grown for 24 h in culture medium, washed twice with HEPES/EDTA and treated with bFGF in EBM-2 medium containing 0.5% FCS for 48 h as indicated. Cells were incubated with MTT solution, lysed and the absorbance was read. MTT activity is expressed as Optical Density and represents mean ± SEM of five independent experiments, ** p < 0.01, * p < 0.05.

    Techniques Used: Derivative Assay, Cell Culture, Incubation, Activity Assay



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    PromoCell rat pulmonary artery endothelial cells rpaec
    Proliferative characteristics of bone marrow-derived EPC . EPC and <t>rPAEC</t> <t>were</t> <t>cultured</t> in the presence of 20% FCS in EBM-2 without further supplements (Figure 3A) or in EBM-2 containing bFGF (Figure 3B). Figure 3A shows the differential growth capacities of EPC and mature rPAEC in the presence of EBM-2 supplemented with 20% FCS. Figure 3B shows the proliferative kinetics of EPC cultured in EBM-2 supplemented with 0.5% FCS towards increasing concentrations of bFGF (100 ng/ml to 100 μg/ml). Cells were seeded in 96-well plates, grown for 24 h in culture medium, washed twice with HEPES/EDTA and treated with bFGF in EBM-2 medium containing 0.5% FCS for 48 h as indicated. Cells were incubated with MTT solution, lysed and the absorbance was read. MTT activity is expressed as Optical Density and represents mean ± SEM of five independent experiments, ** p < 0.01, * p < 0.05.
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    Proliferative characteristics of bone marrow-derived EPC . EPC and <t>rPAEC</t> <t>were</t> <t>cultured</t> in the presence of 20% FCS in EBM-2 without further supplements (Figure 3A) or in EBM-2 containing bFGF (Figure 3B). Figure 3A shows the differential growth capacities of EPC and mature rPAEC in the presence of EBM-2 supplemented with 20% FCS. Figure 3B shows the proliferative kinetics of EPC cultured in EBM-2 supplemented with 0.5% FCS towards increasing concentrations of bFGF (100 ng/ml to 100 μg/ml). Cells were seeded in 96-well plates, grown for 24 h in culture medium, washed twice with HEPES/EDTA and treated with bFGF in EBM-2 medium containing 0.5% FCS for 48 h as indicated. Cells were incubated with MTT solution, lysed and the absorbance was read. MTT activity is expressed as Optical Density and represents mean ± SEM of five independent experiments, ** p < 0.01, * p < 0.05.
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    Proliferative characteristics of bone marrow-derived EPC . EPC and <t>rPAEC</t> <t>were</t> <t>cultured</t> in the presence of 20% FCS in EBM-2 without further supplements (Figure 3A) or in EBM-2 containing bFGF (Figure 3B). Figure 3A shows the differential growth capacities of EPC and mature rPAEC in the presence of EBM-2 supplemented with 20% FCS. Figure 3B shows the proliferative kinetics of EPC cultured in EBM-2 supplemented with 0.5% FCS towards increasing concentrations of bFGF (100 ng/ml to 100 μg/ml). Cells were seeded in 96-well plates, grown for 24 h in culture medium, washed twice with HEPES/EDTA and treated with bFGF in EBM-2 medium containing 0.5% FCS for 48 h as indicated. Cells were incubated with MTT solution, lysed and the absorbance was read. MTT activity is expressed as Optical Density and represents mean ± SEM of five independent experiments, ** p < 0.01, * p < 0.05.
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    Proliferative characteristics of bone marrow-derived EPC . EPC and rPAEC were cultured in the presence of 20% FCS in EBM-2 without further supplements (Figure 3A) or in EBM-2 containing bFGF (Figure 3B). Figure 3A shows the differential growth capacities of EPC and mature rPAEC in the presence of EBM-2 supplemented with 20% FCS. Figure 3B shows the proliferative kinetics of EPC cultured in EBM-2 supplemented with 0.5% FCS towards increasing concentrations of bFGF (100 ng/ml to 100 μg/ml). Cells were seeded in 96-well plates, grown for 24 h in culture medium, washed twice with HEPES/EDTA and treated with bFGF in EBM-2 medium containing 0.5% FCS for 48 h as indicated. Cells were incubated with MTT solution, lysed and the absorbance was read. MTT activity is expressed as Optical Density and represents mean ± SEM of five independent experiments, ** p < 0.01, * p < 0.05.

    Journal: Respiratory Research

    Article Title: Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury

    doi: 10.1186/1465-9921-8-50

    Figure Lengend Snippet: Proliferative characteristics of bone marrow-derived EPC . EPC and rPAEC were cultured in the presence of 20% FCS in EBM-2 without further supplements (Figure 3A) or in EBM-2 containing bFGF (Figure 3B). Figure 3A shows the differential growth capacities of EPC and mature rPAEC in the presence of EBM-2 supplemented with 20% FCS. Figure 3B shows the proliferative kinetics of EPC cultured in EBM-2 supplemented with 0.5% FCS towards increasing concentrations of bFGF (100 ng/ml to 100 μg/ml). Cells were seeded in 96-well plates, grown for 24 h in culture medium, washed twice with HEPES/EDTA and treated with bFGF in EBM-2 medium containing 0.5% FCS for 48 h as indicated. Cells were incubated with MTT solution, lysed and the absorbance was read. MTT activity is expressed as Optical Density and represents mean ± SEM of five independent experiments, ** p < 0.01, * p < 0.05.

    Article Snippet: Rat pulmonary artery endothelial cells (rPAEC) were cultured in endothelial culture medium (Promo Cell, Heidelberg, Germany) containing 10% FCS and 2% endothelial cell growth supplement (Promo Cell, Heidelberg, Germany), 1% penicillin/streptomycin solution (Sigma, St. Louis, MO) and kept in a humidified incubator at 37°C in 5% CO 2 . rPAEC were used between passages three and five for all experiments.

    Techniques: Derivative Assay, Cell Culture, Incubation, Activity Assay